Experiments on Vital Staining with Methylene Blue
نویسندگان
چکیده
منابع مشابه
The vital staining of muscle biopsies with methylene blue.
The histological changes of muscle occurring in neuromuscular diseases are often non-specific. They do not always enable us to find out the cause of an atrophy or a degeneration or to assess the part played by a disorder of innervation. Therefore, it would be desirable, when investigating muscle samples taken by biopsy, to be able to study systematically and simultaneously the muscular tissue a...
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The morphological identification of Trypanosoma cruzi is currently considered to have a high specificity, but its sensitivity, which depends on the volume of the sample examined, is rather low. Trypanosome developmental stages suspended in blood, reduviid feces, and culture media are routinely searched for by means of fresh film examination (about 2 microL). High speed centrifugation of blood s...
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A new method is described for vital staining of nervous tissue with methylene blue; it involves impregnation of whole embryos or tissue-fragments with the rongalitreduced dye under anaerobic conditions at pH 5, followed by development of the stain in an oxygenated neutral saline solution. Determinations of the solubility of leucomethylene blue and experiments on vital staining with it and with ...
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Methylene blue staining method was used to distinguish O. viverrini eggs from Haplorchis taichui and Prosthodendrium molenkampi eggs. All eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. The distinct musk-melon-like texture of the O. viverini eggshell surface and the thread-like texture of H. taichui egg...
متن کاملStudies on Vital Staining
When large amounts of brilliant vital red are injected into the blood stream of dogs, the dye is gradually removed from circulation, and most of it is deposited in numerous phagocytic cells which are scattered throughout various organs and tissues. The dye occurs largely in the form of tiny red granules crowded together in the cytoplasm of these cells. If Niagara sky blue, a closely related dye...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 1953
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.s3-94.26.113